RPMI Medium 1640 細胞培養(yǎng) solarbio

RPMI Medium 1640 細胞培養(yǎng) solarbio

RPMI Medium 1640，含L-谷氨酰胺、酚紅，不含丙酮酸鈉、HEPES

紅細胞裂解液的配置方法

取100 μl抗凝全血，加2 ml應用液3#，混勻15～20 s；加2 ml應用液2#，混勻15～20 s；加4 ml應用液1#，混勻15～20 s；300×g離心2 min，棄上清；收集沉淀，加PBS緩沖液，制成白細胞懸液，備用。（這種紅細胞裂解法滿足了白細胞流式細胞儀檢測分選要求，價格低廉，是一種簡便快捷、經濟實用的白細胞純化分選方法。）

紅細胞裂解液的注意事項

1、本裂解液為無菌產品，分離細胞用于細胞培養(yǎng)時請注意無菌操作。

2、為了您的安全與健康，請穿實驗服并戴一次性手套操作。

3、 儲存液儲存于2-8°C不能超過半年(六個月)。

4、使用時根據(jù)需要稀釋,每次用都應用去離子水新鮮配制工作液,當日用不完的可廢棄。

北京索萊寶科技有限公司為紅細胞裂解液生產廠家，貨號為R1010，常賣規(guī)格為100ml 、500ml。索萊寶生產的紅細胞裂解液是利用細胞內外存在鹽離子濃度差而導致細胞膜脹破的原理來裂解無核紅細胞的。產品經過無菌處理，主要用于經酶消化分散的組織細胞的分離純化，淋巴細胞的分離純化以及組織細胞蛋白與核酸提取等實驗中紅細胞的去除。

相關文獻：

《c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma》 作者:Shangfeng Li, Shengkai Zhang， Jie Chen 期刊:Cancer gene therapy 影響因子:4.681 PMID:30607006
《Suberoylanilide hydroxamic acid inhibits proliferation and promotes apoptosis of mouse myeloma Sp2/0 cells through activating p53/caspase-3 signaling》 作者:Yanliang Bai, Lei Huo, Chenyu Zhang, Mingyue Shi, Na Fang, Shaoping Ji, Kai Sun 期刊:INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 影響因子:1.875 PMID:
《Gecko Active Components Regulates Endoplasmic Reticulum Stress to Induce the Apoptosis of KYSE150 Cells through PERK Pathway》 作者:Yimeng Duan, Lengxin Duan, Zeyue Huang, Bingbing Wang, Jiangang Wang 期刊:Journal of Biosciences and Life Sciences 影響因子:0.552 PMID:
《RNA-binding protein RBM6 as a tumor suppressor gene represses the growth and progression in laryngocarcinoma》 作者:Qiwei Wang,Fang Wang,Waisheng Zhong,Hang Ling,Jixuan Wang,Jie Cui,Tao Xie,Senli Wen,Jie Chen 期刊:Gene 影響因子:2.638 PMID:30772516
《Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway》 作者:Panpan Zhang,Lingyue Hua,Huan Hou, Xingyue Du,Zhiqiang He, Menghan Liu, Xiaojuan Hu, Nianlong Yan 期刊:International Journal of Molecular Medicine 影響因子:2.928 PMID:30272329
《Downregulation of transcription factor EB inhibits the growth and metastasis of colorectal carcinomas》 作者:Jin-Zhe Chang,Shu-Dong Chen,Hui Zheng,Hua-Ping Zhang 期刊:European Journal of Inflammation 影響因子:0.733 PMID:
《Circular RNA hsa_circ_0002024 suppresses cell proliferation, migration, and invasion in bladder cancer by sponging miR-197-3p》 作者:Yongjun Jiang, Tingran Wei, Wei Li, Ruihua Zhang, and Maogang Chen 期刊:American journal of Translational Research 影響因子:3.266 PMID:30972190
《Effect of microRNA‐128 on cisplatin resistance of glioma SHG‐44 cells by targeting JAG1》 作者:Dong‐Ye Yi， Qing Su， Fang‐Cheng Zhang， Peng Fu， Qing Zhang， Yong‐Cun Cen， Hong‐Yang Zhao，Wei Xiang 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:29091297
《miR‐377‐5p inhibits lung cancer cell proliferation, invasion, and cell cycle progression by targeting AKT1 signaling》 作者:Han Wu，Haiyan Liu,Wenjie Liu,Yongli Shi,Dawei Bao 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30485528
《Activity of coumarin against Candida albicans biofilms》 作者:K.Xu  J.L.Wang  M.P.Chu  C.Jia 期刊:Journal de Mycologie Medicale 影響因子:1.479 PMID:30606640
《Gallic acid has anticancer activity and enhances the anticancer effects of cisplatin in non?small cell lung cancer A549 cells via the JAK/STAT3 signaling pathway》 作者:Tingxiu Zhang, Lijie Ma, Pengfei Wu, Wei Li, Ting Li, Rui Gu, Xiaoping Dan, Zhiwei Li, Xianming Fan, Zhenliang Xiao 期刊:Oncology Reports 影響因子:3.041 PMID:30747218
《c-Myc, RMRP, and miR-34a-5p form a positive-feedback loop to regulate cell proliferation and apoptosis in multiple myeloma》 作者:Xichun Xiao,Yueli Gu,Genjie Wang,Shuxia Chen 期刊:International Journal of Biological Macromolecules 影響因子:4.784 PMID:30389523
《Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis》 作者:Xin Chen, Nuobei Zhang 期刊:Cell Biology International 影響因子:2.127 PMID:30488993
《Knockdown of USP28 enhances the radiosensitivity of esophageal cancer cells via the c‐Myc/hypoxia‐inducible factor‐1 alpha pathway》 作者:Zhang Weili， Liu Zhikun，Wang Jianmin， Tian Qingbao 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30206969
《Circulating exosomes activate dendritic cells and induce unbalanced CD4+ T cell differentiation in Hashimoto thyroiditis》 作者:Xuejiao Cui, Yongping Liu, Shuo Wang, Na Zhao, Jing Qin, Yushu Li,Chenling Fan, Zhongyan Shan, Weiping Teng 期刊:The Journal of Clinical Endocrinology & Metabolism 影響因子:5.605 PMID:31199456
《LPS?induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF?κB, STAT3 or AP?1 activation》 作者:Xuefang Liu， Sugai Yin， Yulong Chen， Yaosong Wu， Wanchun Zheng， Haoran Dong， Yan Bai， Yanqin Qin， Jiansheng Li， Suxiang Feng， Peng Zhao 期刊:Molecular Medicine Reports 影響因子:1.851 PMID:29393460
《Therapeutic effects of Hedyotis diffusa Willd in a COPD mouse model challenged with LPS and smoke》 作者:Renping Liu，Peihong Wang，Caiqing Wu，Juan Chen，Chengxin Li，Yongtao Xie，Qi Wang，Jianming Liu，Huan He，Jing Zhu 期刊:Experimental
   and Therapeutic Medicine 影響因子:1.41 PMID:29545859
《Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling》 作者:Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rongkun Li, Fang Fang, Guangang Tian ,Zhigang Zhang 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:25738465
《TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression》 作者:L Sun, Z Dong, H Gu, Z Guo, Z Yu 期刊:Cancer Management and Research 影響因子:3.702 PMID:30697069
《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:
《Evaluation of the cytotoxicity of cigarette smoke total particulate matter using three in vitro assays and two types of cells》 作者:Xiang Li, Bin Peng, Cong Nie, Pingping Shang & Huimin Liu 期刊:Journal Toxicology Mechanisms and Methods 影響因子:1.367 PMID:23193988
《Ethylenecarbodiimide-fixed splenocytes carrying whole islet antigens decrease the incidence of diabetes in NOD mice via down-regulation of effector memory T cells and autoantibodies》 作者:Jiaqi Zou, Xinpu Gao, Tengli Liu, Rui Liang, Yaojuan Liu, Guanqiao Wang, Le Wang, Na Liu, Peng Sun, Zhiping Wang, Shusen Wang, Zhongyang Shen 期刊:Endocrine Journal 影響因子:1.911 PMID:29998909
《SIRT1 expression regulates the transformation of resistant esophageal cancer cells via the epithelial-mesenchymal transition》 作者:Tiantian Qin,Weihua Liu,Junfeng Huo,Leilei Li,Xueyan Zhang,Xiaoli Shi,Jinlei Zhou,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:29656187
《Targeting the Bcl-2 family and P-glycoprotein reverses paclitaxel resistance in human esophageal carcinoma cell line》 作者:Xiaoli Shi,Yinhui Dou,Kairui Zhou,Jinling Huo,Tengjiao Yang,Tiantian Qin,Weihua Liu,Saiqi Wang,Dongxiao Yang,Liming Chang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:3.457 PMID:28441715
《High incidence of coding gene mutations in mitochondrial DNA in esophageal cancer》 作者:Zong?Wen Liu, Zhen?Jiang Guo, A?Lan Chu, Yan Zhang, Bing Liang, Xing Guo, Ting Chai, Rui Song, Ge Hou, Jin?Jin Yuan 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:28990096
《在前DC到成熟DC的分化過程中表型和分泌功能的動態(tài)變化》 作者:王晶（汪晶）立博釗（趙利波）升昌（昌盛）Chang-sheng Ming （明長生）楊軍（楊軍） 期刊:華中科技大學學報 影響因子:0.38 PMID:
《Simultaneous determination of saikosaponins in Bupleurum Radix from different locations by high performance liquid chromatography- charged aerosol detection (HPLC-CAD) method and its immunomodulation effects on mouse splenocytes》 作者:Lina Wang, Bin Chen, Wei Wang, Nan Xu and Tianzhu Jia* 期刊:academic Journals 影響因子: PMID:
《Knockdown of hsa_circ_0023028 inhibits cell proliferation, migration, and invasion in laryngeal cancer by sponging miR-194-5p》 作者:Xiaofeng Chen, Xiaoqing Su, Chuansai Zhu, Jing Zhou 期刊:Bioscience Reports 影響因子:2.535 PMID:31123169
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影響因子: PMID:
《Abnormal expression and mutation of the RBPJ gene may be involved in CD59? clonal proliferation in paroxysmal nocturnal hemoglobinuria》 作者:Liyan Li, Hui Liu, Honglei Wang ,Zhaoyun Liu ,Yingying Chen, Lijuan Li ,Jia Song, Guojin Wang, Rong Fu 期刊:Experimental and Therapeutic Medicine 影響因子:1.448 PMID:31086586
《Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma》 作者:Hui Qin,Yunyun Li, Hongyan Zhang,Feng Wang, Hongliu He, Xue Bai,and Shanshan Li 期刊:OncoTargets and Therapy 影響因子:3.046 PMID:30881043
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei  Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影響因子:2.502 PMID:31089713
《In vitro and in vivo studies of antiosteosarcoma activities of formononetin》 作者:Wei Hu , Xianpei Wu,  Jiandong Tang , Niansu Xiao , Guoping Zhao,  Li Zhang , Luanhai Ou 期刊:Journal of Cellular Physiology 影響因子:4.522 PMID:30790283
《Trichomonas vaginalis triggers the release of THP-1 extracellular traps》 作者:Li Fei,Wei Zhengkai,Jiang Weina,Cao Lili,Gao Yuhang,Yang Zhengtao,Li Jianhua,Yu BiaoZhang, Xichen,Gong Pengtao 期刊:Parasitology Research 影響因子:2.067 PMID:30426227

 

RPMI Medium 1640 細胞培養(yǎng) solarbio